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Caspase 3 antibody (N-Term)

CASP3 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IHC (p), IP Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN219227

Datasheet as PDF

Target See all Caspase 3 (CASP3) Antibodies

Caspase 3 (CASP3)
Binding Specificity
19

16

16

14

13

12

12

10

9

8

8

8

7

6

6

6

5

5

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
212

117

74

17

11

10

9

8

7

5

5

4

3

3

2

2

1

1
Human, Mouse, Rat
Host
225

19

4

2

2
Rabbit
Compatible Secondaries
Clonality
215

36
Polyclonal
Conjugate
152

19

17

9

8

6

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This Caspase 3 antibody is un-conjugated
Application
184

105

100

57

42

34

29

28

28

27

26

10

10

6

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity

Recognizes human caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17, 19kD). Species cross-reactivity: mouse and rat.

Purification

Protein A purified

Immunogen

Synthetic peptide corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3 (KLH).

Type of Immunogen: Synthetic peptide

Isotype

IgG

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anti-Caspase 3 (CASP3) (AA 220-236), (C-Term) antibody
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CASP3 Reactivity: Human, Mouse, Rat WB, IHC Host: Mouse Monoclonal 5E1 unconjugated

anti-Caspase 3 (CASP3) (pSer150) antibody
CASP3 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

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CASP3 Reactivity: Human, Mouse, Rat, Cow WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Caspase 3 (CASP3) (cleaved) antibody
CASP3 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 100-200) antibody
CASP3 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 29-175) antibody
CASP3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 29-175) antibody
CASP3 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C3 unconjugated

Application Notes

Approved: ELISA, IHC, IHC-P, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:200. Immunoprecipitation: 1:50.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative

Without preservative

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

Caspase 3 (CASP3)

Alternative Name

CASP3 / Caspase 3 (CASP3 Products)

Background

Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID

836

UniProt

P42574

Pathways

Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation