CD59 antibody

Catalog No. ABIN2192125

Product Details anti-CD59 Antibody

Target: CD59

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Sterility: 0.2 μm filtered

Clone: TH9

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CD59 antibody (ABIN2192125)

Rogers, Gasque, Piddlesden, Okada, Holers, Morgan: "Expression and function of membrane regulators of complement on rat astrocytes in culture." in: Immunology, Vol. 88, Issue 1, pp. 153-61, (1996) (PubMed).

Hughes, Meri, Davies, Williams, Morgan: "Immunolocalization and characterization of the rat analogue of human CD59 in kidney and glomerular cells." in: Immunology, Vol. 80, Issue 3, pp. 439-44, (1994) (PubMed).

Hughes, Piddlesden, Williams, Harrison, Morgan: "Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement." in: The Biochemical journal, Vol. 284 ( Pt 1), pp. 169-76, (1992) (PubMed).

Target Details for CD59

Target: CD59

Alternative Name: CD59 Glycoprotein (CD59 Products)

Background: The monoclonal antibody TH9 recognizes rat CD59. Rat CD59 was previously known as rat inhibitory protein (RIP) and it is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization.CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes. In rat, CD59 is expressed on vacular endothelium and circulating cells. In the central nervous system (CNS) of the rat, CD59 is expressed on the Schwann sheath of peripheral nerve fibres and on ependymal cells, but not on glial cells and neurons in the CNS. Rat astrocytes in vitro express CD59 on its surface. CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells. Aliases Membrane attack complex inhibition factor, MACIF, MAC-inhibitory protein, Protectin, CD59 Immunogen Partially purified (FPLC) rat CD59 prep (RIP)

Pathways: Complement System