CD14 antibody

Catalog No. ABIN2192076

Product Details anti-CD14 Antibody

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Inhibition Assay (InhA)

Sterility: 0.2 μm filtered

Clone: 18D11

Application Details

Application Notes: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of human sCD14 to be inactivated.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CD14 antibody (ABIN2192076)

Tunheim, Schjetne, Fredriksen, Sandlie, Bogen: "Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes." in: Journal of leukocyte biology, Vol. 77, Issue 3, pp. 303-10, (2005) (PubMed).

Wang, Jørgensen, Ellingsen, Almiöf, Thiemermann, Foster, Aasen, Solberg: "Peptidoglycan primes for LPS-induced release of proinflammatory cytokines in whole human blood." in: Shock (Augusta, Ga.), Vol. 16, Issue 3, pp. 178-82, (2001) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: The monoclonal antibody 18D11 recognizes human monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells. The monoclonal antibody 18D11 reacts with both soluble and membrane CD14. The monoclonal antibody 18D11 is useful for inhibition of biological activity of sCD14, it inhibits LPS induced TNF production. The monoclonal antibody is cross reactive with pig CD14. Together with a C5a neutralizing monoclonal antibody, 18D11 blocks sepsis in a pig model.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades