CD46 antibody

Catalog No. ABIN2191979

Product Details anti-CD46 Antibody

Target: CD46

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Sterility: 0.2 μm filtered

Clone: M177

Application Details

Application Notes: For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive CD46 is expressed on every cell and tissue control Negative Erythrocytes control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-CD46 antibody (ABIN2191979)

Kato, Ohgimoto, Sharma, Kurazono, Ayata, Komase, Takeda, Takeuchi, Ihara, Ogura: "Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM." in: Vaccine, Vol. 27, Issue 29, pp. 3838-48, (2009) (PubMed).

Loré, Adams, Havenga, Precopio, Holterman, Goudsmit, Koup: "Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1721-9, (2007) (PubMed).

Kurita-Taniguchi, Hazeki, Murabayashi, Fukui, Tsuji, Matsumoto, Toyoshima, Seya: "Molecular assembly of CD46 with CD9, alpha3-beta1 integrin and protein tyrosine phosphatase SHP-1 in human macrophages through differentiation by GM-CSF." in: Molecular immunology, Vol. 38, Issue 9, pp. 689-700, (2002) (PubMed).

Iwata, Seya, Yanagi, Pesando, Johnson, Okabe, Ueda, Ariga, Nagasawa: "Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15148-52, (1995) (PubMed).

Seya, Hara, Matsumoto, Akedo: "Quantitative analysis of membrane cofactor protein (MCP) of complement. High expression of MCP on human leukemia cell lines, which is down-regulated during cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 1, pp. 238-45, (1990) (PubMed).

Target Details for CD46

Target: CD46

Alternative Name: Cd46, Membrane Cofactor Protein (CD46 Products)

Background: The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity. It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain Aliases MCP, TLX, AHUS2, MIC10, TRA2.10, MGC26544, complement regulatory protein, trophoblast leucocyte common antigen Immunogen Purified MCP

Pathways: Regulation of Actin Filament Polymerization