CD13 antibody

Catalog No. ABIN2191832

Product Details anti-CD13 Antibody

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sterility: 0.2 μm filtered

Clone: ER-BMDM1

Application Details

Application Notes: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CD13 antibody (ABIN2191832)

Leenen, Radosevi?, Voerman, Salomon, van Rooijen, Klatzmann, van Ewijk: "Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2166-73, (1998) (PubMed).

Allaerts, Salomon, Leenen, van Wijngaardt, Jeucken, Ruuls, Klatzmann, Drexhage: "A population of interstitial cells in the anterior pituitary with a hematopoietic origin and a rapid turnover: a relationship with folliculo-stellate cells?" in: Journal of neuroimmunology, Vol. 78, Issue 1-2, pp. 184-97, (1997) (PubMed).

Leenen, Melis, Kraal, Hoogeveen, Van Ewijk: "The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity." in: European journal of immunology, Vol. 22, Issue 6, pp. 1567-72, (1992) (PubMed).

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Abstract: ANPEP Products

Background: The monoclonal antibody ER-BMDM1 is directed against a 160- kDa cell membrane-associated antigen with aminopeptidase N activity. Aminopeptidase N (APN) is also known as Alanyl Aminopeptidase (ANPEP), CD13 antigen or membrane protein p161. The protein consists of a short cyplasmic tail, a transmembrane region, a Ser/Thr region, and a zinc metalloproteinase domain. Widely expressed in many cells, tissues, and species, aminopeptidase N cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. It has putative involvement in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis and neurotransmitter degradation. The antigen recognized by the mocnoclonal antibody ER-BMDM1 is present on various mouse macrophage and dendritic cell subpopulations as well as on microvillous epithelia. The ER-BMDM1 antigen is expressed at increasing levels upon maturation of macrophages. Therefore the monoclonal antibody ER-BMDM1 is a useful marker for the identification of aminopeptidase N positive macrophages and dendritic cells in mouse, the expression of the ER- BMDM1 antigen arises after the monocytic stage of differentation: bone marrow cells and peripheral blood monocytes are ER-BMDM1 negative, whereas mature macrophages express the ER-BMDM1 antigen. A subpopulation of dendrocytes and their in vitro equivalents, the dendritic cells, are also ER- BMDM1 positive.

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones