TNF alpha antibody

Catalog No. ABIN2191760

Product Details anti-TNF alpha Antibody

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Cross-Reactivity (Details): Cross reactivity: Chinese hamster TNF-α : Yes, Receptor-bound mouse TNF-α : No

Sterility: 0.2 μm filtered

Clone: V1q

Isotype: IgD

Application Details

Application Notes: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Monocytes/macrophages control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-TNF alpha antibody (ABIN2191760)

Rajashekhar, Grow, Willuweit, Patterson, Clauss: "Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation." in: Physiological genomics, Vol. 31, Issue 1, pp. 104-13, (2007) (PubMed).

Demjen, Klussmann, Kleber, Zuliani, Stieltjes, Metzger, Hirt, Walczak, Falk, Essig, Edler, Krammer, Martin-Villalba: "Neutralization of CD95 ligand promotes regeneration and functional recovery after spinal cord injury." in: Nature medicine, Vol. 10, Issue 4, pp. 389-95, (2004) (PubMed).

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." in: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, (2000) (PubMed).

Echtenacher, Falk, Männel, Krammer: "Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3762-6, (1991) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor alpha (TNF alpha Products)

Background: The monoclonal antibody V1q recognizes mouse tumor necrosis factor alpha (TNF-α). TNF-α is the prototype cytokine of the family of TNF-related ligands, which are based on structural and functional homologies. TNF-α is synthesized as type II transmembrane protein. TNF-α can be recognized by two different membrane receptors, namely TNF-R1 and TNF-R2. TNF-α is present in a membrane-bound (tmTNF) as well as soluble form (sTNF). The membrane-bound form of TNF-α is recognized by both TNF receptors with high affinity, whereas the soluble form is recognized more superiorly by TNF-R1. TNF-α is produced by many different cell types including macrophages, T lymphocytes, NK cells, neutrophils and endothelial cells. Cells differ in the expression of the two TNF-receptors and sTNF versus tmTNF, respectively. TNF-α, a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions. TNF-α was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-α has been implied as central mediator in shock induced by gram negative micro-organisms. TNF-α induces on its turn the production of many other cytokines. Furthermore, TNF-α has been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. The monoclonal antibody V1q recognizes both natural and recombinant TNF-α and shows neutralizing activity. Aliases TNF, TNF-SF2, DIF, cachectin, TNF-α, tumor necrosis factor ligand superfamily member 2 Immunogen Cytotoxin purified from conA induced T cell clone 29

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome