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BCR antibody (pTyr177)

BCR Reactivity: Human, Mouse WB, IHC, FACS, IHC (p) Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN219125

Datasheet as PDF

Target See all BCR Antibodies

BCR (Breakpoint Cluster Region (BCR))
Binding Specificity
29

16

8

4

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr177
Reactivity
93

52

25

4

2

2

1

1

1

1
Human, Mouse
Host
86

4

4
Rabbit
Compatible Secondaries
Clonality
89

5
Polyclonal
Conjugate
54

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This BCR antibody is un-conjugated
Application
65

35

31

26

26

20

18

14

9

6

5

4

3

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

Recognizes endogenous levels of human Bcr and Bcr-Abl only when phosphorylated at tyrosine 177. Species cross-reactivity: Mouse.

Purification

Immunoaffinity purified

Immunogen

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr177 of human Bcr. Molecular Weight:160kD (Bcr), 210kD (Bcr-Abl).

Type of Immunogen: Synthetic phospho-peptide

Isotype

IgG

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anti-Breakpoint Cluster Region (BCR) (pTyr177) antibody
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BCR Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Breakpoint Cluster Region (BCR) (pTyr177) antibody
BCR Reactivity: Human WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Breakpoint Cluster Region (BCR) (pTyr360) antibody
BCR Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo (1:50), IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50. Flow Cytometry: 1:50.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

BCR (Breakpoint Cluster Region (BCR))

Alternative Name

CML / BCR (BCR Products)

Background

Name/Gene ID: BCR
Subfamily: BCR
Family: Protein Kinase

Synonyms: BCR, Acute lymphocytic leukemia, ALL, BCR1, BCR/FGFR1 chimera protein, Breakpoint cluster region, Chronic myeloid leukemia, D22S11, CML, D22S662, Philadelphia chromosome, PHL, FGFR1/BCR chimera protein

Molecular Weight

160kDa (Bcr), 210kDa (Bcr-Abl).

Gene ID

613

UniProt

P11274

Pathways

Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling