TNF alpha antibody
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- Target See all TNF alpha Antibodies
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reactivity
- Human
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This TNF alpha antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kD cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells. Is reported to act as a blocking antibody.
- Purification
- Protein G purified
- Immunogen
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Recombinant TNF alpha
Type of Immunogen: Recombinant protein - Clone
- MP9-20A4
- Isotype
- IgG1
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha Reactivity: Human, Rat IHC, FACS, IF, StM Host: Mouse Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha Reactivity: Mouse WB Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Mouse Monoclonal C1 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 71-234) antibodyTNF alpha Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha Reactivity: Human, Mouse, Rat, Pig, Rabbit WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Approved: ELISA (5 - 10 μg/mL), Flo, IHC, IHC-Fr, WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- TNF alpha (TNF alpha Products)
- Background
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Name/Gene ID: TNF
Family: TNF
Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2 - Gene ID
- 7124
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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