FAS antibody

Catalog No. ABIN215840

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: Recognizes the human cell surface antigen Fas (~43kD) expressed in various human cells, including myeloid cells, T lymphoblastoid cells and diploid fibroblasts. This antibody does not recognize TNF and does not cross-react with mouse Fas.

Purification: Immunoaffinity purified

Immunogen: Human diploid fibroblast cell line FS-7.

Clone: CH11

Isotype: IgM

Application Details

Application Notes: Approved: Flo, ICC, WB

Usage: Suitable for use in Flow Cytometry, Western Blot, and Immunocytochemistry. Apoptosis: >15 ng/mL maximally induced apoptosis of human Jurkat cells with 80-90 % mortality after 24 hours of treatment. This lot has an ID50 of 1.0 ng/mL in apoptosis assays. Western Blot: 0.5-2 mg/mL. Immunocytochemistry: 5-10 mg/mL o. Flow cytometry: Use 20 mg/mL. Can be used for activation studies.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Background: Name/Gene ID: FAS
Family: TNF Receptor

Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity