p21 antibody (AA 58-77)

Catalog No. ABIN215393

Product Details anti-p21 Antibody

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Binding Specificity: AA 58-77

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.

Purification: Protein A purified

Immunogen: Full length recombinant human p21Cip1/WAF1.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (2 μg/mL), IF (1 - 5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB

Usage: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 μg/mL. Flow Cytometry: 2 μg/mL. Western Blot (chemiluminescence): 0.1-1 μg/mL. Immunohistochemistry (frozen/paraffin): 5 μg/mL. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 μg/IP reaction. Immunoprecipitates p21 but not associated proteins.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied s a liquid in PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A / WAF1 / p21 (CDKN1A Products)

Background: Name/Gene ID: CDKN1A

Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy