IL-24 antibody (AA 100-150)

Catalog No. ABIN213735

Product Details anti-IL-24 Antibody

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Binding Specificity: AA 100-150

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-24 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Brand: IHC-plus™

Specificity: The amino acid sequence used as immunogen is found in many of the IL-24 isoforms, including isoforms of 219, 207, 206, 154, 143 and 120 amino acids.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon (93%) Marmoset (86%).

Purification: Protein G purified

Immunogen: Amino acids 123-136 (KNYHNRTVEVRTLK)of human IL-24 were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon (93%), Marmoset (86%).

Isotype: IgG

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (10 μg/mL), WB (0.5 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL-24

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Alternative Name: IL24 (IL24 Products)

Background: Name/Gene ID: IL24
Family: Interleukin

Synonyms: IL24, FISP, IL10B, IL-24, IL-4-induced secreted protein, Interleukin 24, Interleukin-24, Melanocyte-associated Mda-7, Mob-5, ST16, Mda-7, MDA7, MOB5, C49A

Gene ID: 11009

UniProt: Q13007

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagy