Histone H4 antibody (acLys4, acLys7, acLys11, acLys15)

Catalog No. ABIN211196

Product Details anti-Histone H4 Antibody

Target: Histone H4

Binding Specificity: AA 2-19, acLys4, acLys7, acLys11, acLys15

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Recognizes acetylated histone H4 of approximately 10kD of human and tetrahymena. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins.

Purification: Protein A purified

Immunogen: KLH-conjugated peptide AGG[Ac]KGG[AcK]GMG[AcK]VGA[AcK]RHS-C corresponding to the amino acids 2-19 of Tetrahymena histone H4.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 μg/mL will detect acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate, a previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. Immunoprecipitation: 5 μg will immunoprecipitate acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours. Immunocytochemistry: 5-10 μg/mL gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1 % paraformaldehyde in PBS. Western Blot Analysis. Acid-extracted proteins from normal HeLa cells and HeLa cells treated with 5 mM sodium butyrate for 24 hours were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetylated Histone H4 (1 μg/mL). Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products