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CD8 alpha antibody

CD8A Reactivity: Rat FACS, IHC, WB, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal unconjugated

Catalog No. ABIN210181

Datasheet as PDF

Target See all CD8 alpha (CD8A) Antibodies

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
Reactivity
178

106

46

45

13

7

4

2

2

1

1

1

1

1

1

1

1
Rat
Host
149

123

91
Mouse
Compatible Secondaries
Clonality
262

96

2
Monoclonal
Conjugate
123

41

29

25

24

8

8

8

7

6

5

5

5

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 alpha antibody is un-conjugated
Application
289

113

82

60

58

46

32

20

15

14

13

11

7

6

6

6

5

3

3

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

Recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies. XXX recommend the use of MCA48XZ for this purpose. * This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (3) This product is routinely tested in flow cytometry on rat splenocytes.

Purification

Protein G purified

Immunogen

Rat thymocyte membrane glycoproteins.

Type of Immunogen: Membrane extract

Isotype

IgG1

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CD8A Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 8H8A8 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC, Func Host: Rat Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC Host: Rat Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody (FITC)
CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 FITC

anti-CD8a Molecule (CD8A) antibody (PE)
CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 PE

anti-CD8a Molecule (CD8A) (AA 51-150) antibody
CD8A Reactivity: Human, Rat, Chicken FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) (AA 22-182) antibody
CD8A Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 6A9A5 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, StM, ISt Host: Mouse Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A Reactivity: Mouse, Rat FACS, IHC, WB, ELISA, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo (1:100), IHC, IHC-Fr, IHC-P, IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.

Comment

Target Species of Antibody: Rat

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 0.09 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Target

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name

CD8A / CD8 alpha (CD8A Products)

Background

Name/Gene ID: CD8A

Synonyms: CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2

Gene ID

925

Pathways

TCR Signaling