CD28 antibody (FITC)

Catalog No. ABIN207228

Product Details anti-CD28 Antibody (FITC)

Target: CD28

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.

Purification: Protein G purified

Immunogen: Alternate immunization with A20 cells expressing mCD28 and recombinant mCD28Ig.

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (1:1 - 1:5)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Background: Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response