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CD14 antibody (Biotin)

CD14 Reactivity: Human FACS, IF Host: Mouse Monoclonal MEM-15 Biotin

Catalog No. ABIN207197

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Reactivity
231

38

37

25

25

23

23

23

18

16

15

15

14

14

13

10

8

7

6

5

5

2

1

1
Human
Host
205

52

16

3

1
Mouse
Compatible Secondaries
Clonality
223

54
Monoclonal
Conjugate
116

33

23

19

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is conjugated to Biotin
Application
209

82

62

50

49

45

21

15

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity

Reacts with CD14, a 53-55 kD GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.

Purification

Protein A purified

Immunogen

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Type of Immunogen: Protein extract

Clone

MEM-15

Isotype

IgG1

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CD14 Reactivity: Human FACS, WB, IF, IHC (p) Host: Rabbit Polyclonal RB14105 unconjugated

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CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reactivity: Human IHC, ELISA, ICC Host: Mouse Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Sheep, Dog, Rabbit WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo (1:1000), IF (1:1000)

Usage: Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by th The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freeze thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID

929

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades