Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Blocking Antibody (Inhibition)
- Specificity
- Reacts with human vimentin, a 57 kD intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
- Purification
- Protein A purified
- Immunogen
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Recombinant human vimentin.
Type of Immunogen: Recombinant protein - Clone
- VI-RE-1
- Isotype
- IgG1
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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Approved: ELISA, Flo (1 - 10 μg/mL), ICC, Inhb, WB (1 - 2 μg/mL)
Usage: Flow Cytometry: Recommended dilution - 1-10 μg/mL. Western Blotting: Recommended dilution - 1-2 μg/mL, overnight in 4 degrees C. Positive control - LEP-19 cell lysate. Negative control - 3T3 mouse cell line. Sample preparation: Resuspend approx. 50 mil. c
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 15 mM sodium azide, approx, pH 7.4.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Background
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Name/Gene ID: VIM
Synonyms: VIM, Vimentin - Gene ID
- 7431
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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