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CD80 antibody

CD80 Reactivity: Mouse FACS, IHC, IHC (fro) Host: Rat Monoclonal RM80 unconjugated

Catalog No. ABIN206612

Datasheet as PDF

Target See all CD80 Antibodies

CD80
Reactivity
144

95

45

6

6

6

1

1

1
Mouse
Host
92

73

20

17

10

2

1
Rat
Compatible Secondaries
Clonality
132

80

2
Monoclonal
Conjugate
93

18

16

15

10

5

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This CD80 antibody is un-conjugated
Application
124

91

67

35

34

22

14

13

13

12

11

10

8

5

4

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Recognizes mouse CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.

Purification

Protein G purified

Immunogen

BCL1 cells expressing CD80.

Clone

RM80

Isotype

IgG2a

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anti-CD80 (CD80) (AA 35-288) antibody
CD80 Reactivity: Human FACS, ELISA, IHC Host: Mouse Monoclonal 1G3D7 unconjugated

anti-CD80 (CD80) antibody
CD80 Reactivity: Mouse, Dog FACS, IP, IHC (fro) Host: Armenian Hamster Monoclonal 16-10A1 unconjugated

anti-CD80 (CD80) (AA 38-246) antibody
CD80 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD80 (CD80) (Extracellular Domain) antibody
CD80 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-233 unconjugated

anti-CD80 (CD80) (AA 251-321) antibody
CD80 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD80 (CD80) (Extracellular Domain) antibody (FITC)
CD80 Reactivity: Human FACS Host: Mouse Monoclonal MEM-233 FITC

anti-CD80 (CD80) (Extracellular Domain) antibody (PerCP)
CD80 Reactivity: Human FACS Host: Mouse Monoclonal MEM-233 PerCP

anti-CD80 (CD80) (Extracellular Domain) antibody (APC)
CD80 Reactivity: Human FACS Host: Mouse Monoclonal MEM-233 APC

anti-CD80 (CD80) (Extracellular Domain) antibody (PE)
CD80 Reactivity: Human FACS Host: Mouse Monoclonal MEM-233 PE

anti-CD80 (CD80) antibody
CD80 Reactivity: Human ELISA, IHC, ICC Host: Mouse Monoclonal 2A2 unconjugated

Application Notes

Approved: Flo (1:10 - 1:50), IHC, IHC-Fr

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request.

Comment

Target Species of Antibody: Mouse

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

CD80

Alternative Name

CD80 / B7 (CD80 Products)

Background

Name/Gene ID: CD80

Synonyms: CD80, Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CD80 antigen, LAB7, CD80 Molecule, CTLA-4 counter-receptor B7.1

Gene ID

941

UniProt

P33681

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints