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Vimentin antibody

VIM Reactivity: Human, Rat, Chicken WB, IHC, FACS, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal unconjugated

Catalog No. ABIN204914

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Reactivity
305

120

117

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Rat, Chicken
Host
158

130

11

6

6

5

2
Mouse
Compatible Secondaries
Clonality
172

145
Monoclonal
Conjugate
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
263

115

104

97

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.

Purification

Protein G purified

Immunogen

Vimentin.

Type of Immunogen: Purified protein

Isotype

IgG1

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VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)

Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer,  pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C,-20 °C

Storage Comment

4°C or -20°C, Avoid freeze-thaw cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gene ID

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis