CD58 antibody (Biotin)

Catalog No. ABIN204009

Product Details anti-CD58 Antibody (Biotin)

Target: CD58

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD58 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Specificity: Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.

Purification: Protein A purified

Immunogen: Human cytotoxic T cells.

Isotype: IgG1

Application Details

Application Notes: Approved: Ctox, Flo, IHC, IHC-Fr, IP

Usage: Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 mL of anti-CD58/Biotin at 10 mg/mL. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94 % blocked when pre incubated 10 minutes with 20 mL of 0.5 mg/mL anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % glycerol, 0.2 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD58

Target: CD58

Alternative Name: CD58 (CD58 Products)

Background: Name/Gene ID: CD58
Family: Immunoglobulin

Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule

Gene ID: 965

UniProt: P19256