NG2 antibody

Catalog No. ABIN200587

Product Details anti-NG2 Antibody

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Specificity: Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.

Purification: Protein A purified

Immunogen: Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, Func, IF, IP

Usage: Suitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Immunofluorescence Microscopy: Cells in culture. Immunoprecipitation: 1 μg/1 x10⁶. FACS analysis: 1-2 μg/1 x10⁶. Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6. No preservative added. Glycerol free.

Preservative: Azide free

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4 / NG2 (MCSP Products)

Background: Name/Gene ID: CSPG4

Synonyms: CSPG4, MCSP, MEL-CSPG, MSK16, NG2, HMW-MAA, MCSPG

Gene ID: 1464

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process