p53 antibody (AA 20-25)

Catalog No. ABIN198280

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: AA 20-25

Reactivity: Human, Cow, Horse, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies. p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancerrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G purified

Immunogen: Recombinant human p53 ( AA 20-25 ).

Type of Immunogen: Recombinant protein

Clone: DO-1

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (1:25), IP, WB (1:1000)

Usage: Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. We recommend the use of 0.01 M citrate buffer for this purpose.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: TP53 / p53 (TP53 Products)

Background: Name/Gene ID: TP53
Family: P53

Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

Gene ID: 7157

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect