IL4 Receptor antibody (Extracellular Domain)

Catalog No. ABIN198262

Product Details anti-IL4 Receptor Antibody

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kD type I single pass transmembrane glycoprotein bearing a single domain. Interleukin-4 Receptor form a heterodimeric complex with the, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/mL in the presence of 0.2 ng/mL of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.

Purification: Protein A purified

Immunogen: Sf 21-derived, recombinant human interleukin 4 receptor, extracellular domain.

Clone: 49-20

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:500 - 1:2000), Flo (1:20 - 1:80), IHC, IHC-P (1:40 - 1:125), WB (1:250 - 1:1000)

Usage: Western Blot: Will detect human IL-4 R under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 10 % trehalose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL4R / CD124 (IL4R Products)

Background: Name/Gene ID: IL4R
Family: Interleukin

Synonyms: IL4R, CD124, CD124 antigen, IL-4RA, IL4R nirs variant 1, IL4RA, Interleukin 4 receptor, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha

Gene ID: 3566

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process