CSF2RA antibody (PE)

Catalog No. ABIN1981895

Product Details anti-CSF2RA Antibody (PE)

Target: CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSF2RA antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 4H1 recognizes an extracellular epitope of human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: CD116-transfected COS cells

Clone: 4H1

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CSF2RA antibody (PE) (ABIN1981895)

Schwarzmaier, Foell, Weinhage, Varga, Däbritz: "Peripheral monocyte functions and activation in patients with quiescent Crohn's disease." in: PLoS ONE, Vol. 8, Issue 4, pp. e62761, (2013) (PubMed).

Koba, Haruta, Matsunaga, Matsumura, Haga, Sasaki, Ikeda, Takamatsu, Nishimura, Senju: "Therapeutic effect of human iPS-cell-derived myeloid cells expressing IFN-? against peritoneally disseminated cancer in xenograft models." in: PLoS ONE, Vol. 8, Issue 6, pp. e67567, (2013) (PubMed).

Huntington, Legrand, Alves, Jaron, Weijer, Plet, Corcuff, Mortier, Jacques, Spits, Di Santo: "IL-15 trans-presentation promotes human NK cell development and differentiation in vivo." in: The Journal of experimental medicine, Vol. 206, Issue 1, pp. 25-34, (2009) (PubMed).

Target Details for CSF2RA

Target: CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))

Alternative Name: CD116 (CSF2RA Products)

Background: Colony stimulating factor 2 receptor alpha subunit,CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5- receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias.,CSF2RA, GMR, CSF2R, SMDP4, GMCSFR

Gene ID: 1438

UniProt: P15509

Pathways: JAK-STAT Signaling