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Ezrin antibody (Thr566)

Name: Ezrin antibody (Thr566)
SKU: ABIN197426
Price: 558 USD
Availability: InStock

EZR Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated

Image no. 1 for anti-Ezrin (EZR) (Thr566) antibody (ABIN197426) (Ezrin antibody (Thr566))

1 image

Catalog No. ABIN197426

Datasheet as PDF

Target See all Ezrin (EZR) Antibodies

Ezrin (EZR)
Binding Specificity
28

16

10

9

9

9

6

5

5

4

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Thr566
Reactivity
140

79

69

9

8

6

6

4

4

3

3

1

1

1

1
Human, Mouse, Rat
Host
130

9

3

2
Rabbit
Compatible Secondaries
Clonality
124

19
Polyclonal
Conjugate
90

6

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1
This Ezrin antibody is un-conjugated
Application
117

55

26

17

16

13

13

13

11

8

3

2

1

1

1

1

1

1

1

1
Western Blotting (WB)

Specificity

This antibody AP02712PU detects endogenous levels of total Ezrin protein.

Purification

Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of Threonine 566 (Y-K-Tp-L-R).

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EZR Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 1E11 unconjugated

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EZR Reactivity: Human WB, IP, IF Host: Rabbit Polyclonal unconjugated

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EZR Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 455-586) antibody
EZR Reactivity: Human, Rat WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

Suitable for use in in Western blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store the antibody (in aliquots) at -20 °C.
Target

Ezrin (EZR)

Alternative Name

Ezrin (EZR Products)

Background

Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cellsSynonyms: Cytovillin, EZR, VIL2, Villin-2, p81

Gene ID

7430

NCBI Accession

NP_001104547

UniProt

P15311

Pathways

Maintenance of Protein Location