The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. SMC1 antibody AP02703PU detects endogenous levels of total SMC1 protein.
Purification
Immunoaffinity chromatography.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human SMC1 around the phosphorylation site of serine 957 (G-S-Sp-Q-G).
Suitable for use in in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody (in aliquots) at -20 °C.
Target
SMC1A
(Structural Maintenance of Chromosomes 1A (SMC1A))