alpha Adducin antibody (Ser726)
-
- Target See all alpha Adducin (ADD1) Antibodies
- alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))
-
Binding Specificity
- Ser726
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This alpha Adducin antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- ADD1 antibody detects endogenous levels of total ADD1 protein.
- Purification
- Immunoaffinity chromatography
- Immunogen
- Synthetic non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-Sp-F-L).
- Top Product
- Discover our top product ADD1 Primary Antibody
-
-
- Application Notes
-
Suitable for use in in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody (in aliquots) at -20 °C.
-
- Target
- alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))
- Alternative Name
- alpha-Adducin (ADD1) (ADD1 Products)
- Background
- Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers alpha/beta, and beta/gamma. The molecular weights for the various isoforms alpha=120 kD, beta=110kD, gamma=80kD. The alpha adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kinases A and C. This protein has been reported to promote the assembly of the spectrinactin network. Polymorphisms in alpha adducin are associated with cardiovascular disease and hypertension. Alpha adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, alpha adducin can regulate membrane ruffling and cell motility.Synonyms: ADDA, Erythrocyte adducin subunit alpha
- Gene ID
- 118
- NCBI Accession
- NP_001110
- UniProt
- P35611
- Pathways
- Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling
-