RPS6KB1 antibody (pThr421)
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- Target See all RPS6KB1 Antibodies
- RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
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Binding Specificity
- pThr421
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RPS6KB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at threonine 421.
- Purification
- Affinity Chromatography using epitope-specific phosphopeptide.
- Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of threonine 421 (P-R-TP-P-V).
- Top Product
- Discover our top product RPS6KB1 Primary Antibody
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anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (AA 1-352) antibody
RPS6KB1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pThr229) antibodyRPS6KB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) antibodyRPS6KB1 Reactivity: Human IHC, ELISA, FACS Host: Mouse Monoclonal 5G9 unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (AA 416-525) antibodyRPS6KB1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2C2 unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (AA 361-525) antibodyRPS6KB1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer424), (pThr421) antibodyRPS6KB1 Reactivity: Human WB, IHC, IF Host: Rabbit Monoclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (AA 91-352) antibodyRPS6KB1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pThr389), (pThr412) antibodyRPS6KB1 Reactivity: Human, Mouse, Rat, Pig WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer371) antibodyRPS6KB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (AA 416-525) antibodyRPS6KB1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1E12 unconjugated
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- Application Notes
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody (in aliquots) at -20 °C.
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- Target
- RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
- Alternative Name
- RPS6KB1 / STK14A (RPS6KB1 Products)
- Background
- Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1
- Gene ID
- 6198
- NCBI Accession
- NP_003152
- UniProt
- P23443
- Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect
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