RPS6 antibody (pSer235)

Catalog No. ABIN196982

Product Details anti-RPS6 Antibody

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Binding Specificity: pSer235

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at Serine 235.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6 (RPS6 Products)

Background: Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33

Gene ID: 6194

NCBI Accession: NP_001001

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly