VASP antibody (pSer239)

Catalog No. ABIN196882

Product Details anti-VASP Antibody

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Binding Specificity: pSer239

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody was affinity-purified from rabbit antiserum by Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. VASP (phospho-Ser239) antibody detects endogenous levels of VASP only when phosphorylated at Serine 239.

Purification: Immunoaffinity Chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human VASP around the phosphorylation site of Serine 239 (K-V-Sp-K-Q).

Application Details

Application Notes: Suitable for use in Western blot (1/500approx. 1/1000) and Immunohistochemistry (1/50approx. 1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP (VASP Products)

Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.Synonyms: Vasodilator-stimulated phosphoprotein

Gene ID: 7408

NCBI Accession: NP_003361

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation