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CD25 antibody (PerCP)

IL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 PerCP

Catalog No. ABIN1959277

Datasheet as PDF

Target See all CD25 (IL2RA) Antibodies

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Reactivity
165

111

36

10

10

5

3

2

2

2

1

1
Human
Host
137

80

77

2

1

1
Mouse
Compatible Secondaries
Clonality
222

70

4
Monoclonal
Conjugate
117

33

28

25

22

11

6

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is conjugated to PerCP
Application
218

86

54

53

44

44

23

18

15

13

8

8

6

6

4

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

Monoclonal antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kD type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.

Purification

Protein A purified

Immunogen

PHA-activated peripheral blood leucocytes.

Type of Immunogen: Cells

Clone

MEM-181

Isotype

IgG1

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IL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 unconjugated

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anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)
IL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 PerCP

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IL2RA Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo

Usage: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.2 % BSA, 0.02 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid prolonged exposure to light.

Storage

4 °C

Storage Comment

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name

IL2RA / CD25 (IL2RA Products)

Background

Name/Gene ID: IL2RA
Family: Interleukin

Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

Gene ID

3559

Pathways

JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation