RFP antibody (Biotin)

Catalog No. ABIN1952568

Product Details anti-RFP Antibody (Biotin)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Chicken

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Purpose: Anti-Red Fluorescent Protein Antibody (Biotin)

Specificity: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis.

Purification: Salt fractionation

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Type of Immunogen: Fusion protein

Application Details

Application Notes: Approved: ELISA (1:10000), WB (1:1000 - 1:3000)

Usage: Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by immunoblotting. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C/-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP / Red Fluorescent Protein (RFP Products)