NeuN antibody

Catalog No. ABIN1929556

Product Details anti-NeuN Antibody

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Clone 1B7 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.

Purification: Affinity purified

Immunogen: N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.

Clone: 1B7

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ICC (1:1000), IF (1:1000), WB (1:1000 - 1:2000)

Usage: The antibody solution can be used at dilutions of 1:1000 or higher in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000-1:2000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 10 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: RBFOX3 / NEUN (RBFOX3 Products)

Background: Name/Gene ID: RBFOX3

Synonyms: RBFOX3

Gene ID: 146713