SOD1 antibody (Internal Region)

Catalog No. ABIN190907

Product Details anti-SOD1 Antibody

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: SOD1

Sequence: SRKHGGPKDE ERH

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 18 kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20 kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9 kDa according to Human NP_000445.1, Mous

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells and the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Additional validation: This antibody has been

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SOD1 antibody (ABIN190907)

Frazziano, Al Ghouleh, Baust, Shiva, Champion, Pagano: "Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4." in: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 2, pp. H197-205, (2014) (PubMed).

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1 (SOD1 Products)

Background: SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) , HGNC:11179, ALS, ALS1, IPOA, SOD , Cu /Zn superoxide dismutase, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase (aa 120-154), superoxide

Gene ID: 6647, 20655, 24786

NCBI Accession: NP_000445

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis