Nanog antibody (C-Term)
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- Target See all Nanog (NANOG) Antibodies
- Nanog (NANOG) (Nanog Homeobox (NANOG))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Nanog antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- NANOG (C Terminus)
- Sequence
- DLFLNYSMNM QPED
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.
- Isotype
- IgG
- Top Product
- Discover our top product NANOG Primary Antibody
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2 μg/mL.
Western Blot: Approx 35 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/mL. An additional band of approx. 37 kDa is observed and is consistent with the obser
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- Alternative Name
- NANOG (NANOG Products)
- Background
- NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
- Gene ID
- 79923
- NCBI Accession
- NP_079141
- Pathways
- Stem Cell Maintenance
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