SMN1 / SMN2 (Internal Region) antibody

Catalog No. ABIN190830

Product Details

Target: SMN1 / SMN2

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: SMN1 / SMN2

Sequence: DESENSRSPG NKSDN

Specificity: This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DESENSRSPGNKSDN, from the internal region of the protein sequence according to NP_075012.1, NP_000335.1, NP_075013.1, NP_075014.1, NP_075015.1, NP_059107.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 28.5 kDa according to NP_075012.1 and NP_075014.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: SMN1 / SMN2

Background: SMN1, survival of motor neuron 1, telomeric, BCD541, SMA1, SMA2, SMA3, SMA4, SMN, SMNT, T-BCD541, gemin 1, SMN2, survival of motor neuron 2, centromeric, BCD541, C-BCD541, SMNC,

Gene ID: 6606, 6607

NCBI Accession: NP_075012, NP_000335, NP_075013, NP_075014, NP_075015, NP_059107