UCHL1 antibody

Catalog No. ABIN1882287

Product Details anti-UCHL1 Antibody

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Hs, Pr, M, Pig

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This UCHL1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.

Clone: 696CT2-1-4

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-UCHL1 antibody (ABIN1882287)

Boudreaux, Maiti, Davies, Das: "Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9117-22, (2010) (PubMed).

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, (2005) (PubMed).

Choi, Levey, Weintraub, Rees, Gearing, Chin, Li: "Oxidative modifications and down-regulation of ubiquitin carboxyl-terminal hydrolase L1 associated with idiopathic Parkinson's and Alzheimer's diseases." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13256-64, (2004) (PubMed).

Day, Hinks, Thompson: "The structure of the human gene encoding protein gene product 9.5 (PGP9.5), a neuron-specific ubiquitin C-terminal hydrolase." in: The Biochemical journal, Vol. 268, Issue 2, pp. 521-4, (1990) (PubMed).

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1 (UCHL1 Products)

Background: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Molecular Weight: 24824

NCBI Accession: NP_004172

UniProt: P09936

Pathways: Feeding Behaviour