p53 antibody

Catalog No. ABIN1882283

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged TP53 protein was used to produced this monoclonal antibody.

Clone: 1227CT355-106-153

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-p53 antibody (ABIN1882283)

Lamb, Crawford: "Characterization of the human p53 gene." in: Molecular and cellular biology, Vol. 6, Issue 5, pp. 1379-85, (1987) (PubMed).

Harris, Brill, Shohat, Prokocimer, Wolf, Arai, Rotter: "Molecular basis for heterogeneity of the human p53 protein." in: Molecular and cellular biology, Vol. 6, Issue 12, pp. 4650-6, (1987) (PubMed).

Harlow, Williamson, Ralston, Helfman, Adams: "Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53." in: Molecular and cellular biology, Vol. 5, Issue 7, pp. 1601-10, (1985) (PubMed).

Zakut-Houri, Bienz-Tadmor, Givol, Oren: "Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells." in: The EMBO journal, Vol. 4, Issue 5, pp. 1251-5, (1985) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: TP53 (TP53 Products)

Background: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Molecular Weight: 43653

NCBI Accession: NP_000537, NP_001119584, NP_001119585, NP_001119586, NP_001119587, NP_001119588, NP_001119589, NP_001119590, NP_001263624, NP_001263625, NP_001263626, NP_001263627, NP_001263628, NP_00126

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect