POLG2 antibody (C-Term)

Catalog No. ABIN1882118

Product Details anti-POLG2 Antibody

Target: POLG2 (Polymerase (DNA Directed), gamma 2, Accessory Subunit (POLG2))

Binding Specificity: AA 348-376, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-376 amino acids from the C-terminal region of human POLG2.

Clone: RB17786

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-POLG2 antibody (ABIN1882118)

Haba, Hatakeyama, Kinoshita, Teramae, Azma, Hatano, Matsuda: "The modulation of vascular ATP-sensitive K+ channel function via the phosphatidylinositol 3-kinase-Akt pathway activated by phenylephrine." in: The Journal of pharmacology and experimental therapeutics, Vol. 334, Issue 2, pp. 673-8, (2010) (PubMed).

Longley, Clark, Yu Wai Man, Hudson, Durham, Taylor, Nightingale, Turnbull, Copeland, Chinnery: "Mutant POLG2 disrupts DNA polymerase gamma subunits and causes progressive external ophthalmoplegia." in: American journal of human genetics, Vol. 78, Issue 6, pp. 1026-34, (2006) (PubMed).

Target Details for POLG2

Target: POLG2 (Polymerase (DNA Directed), gamma 2, Accessory Subunit (POLG2))

Alternative Name: POLG2 (POLG2 Products)

Background: The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2).

Molecular Weight: 54911

NCBI Accession: NP_009146

UniProt: Q9UHN1