NEK6 antibody (N-Term)

Catalog No. ABIN1882104

Product Details anti-NEK6 Antibody

Target: NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))

Binding Specificity: AA 8-39, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK6 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This NEK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-39 amino acids from the N-terminal region of human NEK6.

Clone: RB2965

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-NEK6 antibody (ABIN1882104)

Takeno, Takemasa, Doki, Yamasaki, Miyata, Takiguchi, Fujiwara, Matsubara, Monden: "Integrative approach for differentially overexpressed genes in gastric cancer by combining large-scale gene expression profiling and network analysis." in: British journal of cancer, Vol. 99, Issue 8, pp. 1307-15, (2008) (PubMed).

Belham, Roig, Caldwell, Aoyama, Kemp, Comb, Avruch: "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases." in: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 34897-909, (2003) (PubMed).

Lizcano, Deak, Morrice, Kieloch, Hastie, Dong, Schutkowski, Reimer, Alessi et al.: "Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and ..." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 27839-49, (2002) (PubMed).

Hashimoto, Akita, Hibino, Kohri, Nakanishi: "Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase." in: Biochemical and biophysical research communications, Vol. 293, Issue 2, pp. 753-8, (2002) (PubMed).

Target Details for NEK6

Target: NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))

Alternative Name: NEK6 (NEK6 Products)

Background: NEK6 is a serine/threonine kinase that controls initiation of mitosis. NEK6 is activated during M phase. It is required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis.

Molecular Weight: 35714

NCBI Accession: NP_001138473, NP_001159639, NP_001159640, NP_001159641, NP_001159642, NP_001159643, NP_055212

UniProt: Q9HC98