SQSTM1 antibody (pSer403)

Catalog No. ABIN1881831

Product Details anti-SQSTM1 Antibody

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Binding Specificity: pSer403

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Dot Blot (DB)

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SQSTM1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S403 of human SQSTM1.

Clone: RB41445

Isotype: Ig Fraction

Application Details

Application Notes: DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-SQSTM1 antibody (ABIN1881831)

Visconti, Langston, Alonso, Goodman, Selby, Fraser, Ralston: "Mutations of SQSTM1 are associated with severity and clinical outcome in paget disease of bone." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 25, Issue 11, pp. 2368-73, (2010) (PubMed).

Ding, Ni, Li, Liao, Chen, Stolz, Dorn, Yin: "Nix is critical to two distinct phases of mitophagy, reactive oxygen species-mediated autophagy induction and Parkin-ubiquitin-p62-mediated mitochondrial priming." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27879-90, (2010) (PubMed).

Jain, Lamark, Sjøttem, Larsen, Awuh, Øvervatn, McMahon, Hayes, Johansen: "p62/SQSTM1 is a target gene for transcription factor NRF2 and creates a positive feedback loop by inducing antioxidant response element-driven gene transcription." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22576-91, (2010) (PubMed).

Lau, Wang, Zhao, Villeneuve, Wu, Jiang, Sun, White, Zhang: "A noncanonical mechanism of Nrf2 activation by autophagy deficiency: direct interaction between Keap1 and p62." in: Molecular and cellular biology, Vol. 30, Issue 13, pp. 3275-85, (2010) (PubMed).

Gao, Cao, Bao, Zuo, Xie, Cai, Fu, Zhang, Wu, Zhang, Chen: "Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation." in: Nature cell biology, Vol. 12, Issue 8, pp. 781-90, (2010) (PubMed).

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1 (SQSTM1 Products)

Background: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Molecular Weight: 47687

NCBI Accession: NP_001135770, NP_001135771, NP_003891

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy