MAP1LC3A antibody (pSer12)

Catalog No. ABIN1881524

Product Details anti-MAP1LC3A Antibody

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Binding Specificity: pSer12

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Dot Blot (DB)

Predicted Reactivity: Zf, B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MAP1LC3A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human MAP1LC3A.

Clone: RB30640

Isotype: Ig Fraction

Application Details

Application Notes: DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MAP1LC3A antibody (ABIN1881524)

Scherz-Shouval, Weidberg, Gonen, Wilder, Elazar, Oren: "p53-dependent regulation of autophagy protein LC3 supports cancer cell survival under prolonged starvation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 43, pp. 18511-6, (2010) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Namgoong, Khanal, Cho, Lim, Oh, Kang, Shim, Shim, Yoo, Choi: "The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer." in: The Journal of biological chemistry, Vol. 285, Issue 31, pp. 23829-41, (2010) (PubMed).

Jiang, Cheng, Liu, Peng, Feng: "Protein kinase C inhibits autophagy and phosphorylates LC3." in: Biochemical and biophysical research communications, Vol. 395, Issue 4, pp. 471-6, (2010) (PubMed).

Gao, Cao, Bao, Zuo, Xie, Cai, Fu, Zhang, Wu, Zhang, Chen: "Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation." in: Nature cell biology, Vol. 12, Issue 8, pp. 781-90, (2010) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A (MAP1LC3A Products)

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

NCBI Accession: NP_115903, NP_852610

UniProt: Q9H492, Q9GZQ8

Pathways: Autophagy