CD51/CD61 antibody

Catalog No. ABIN187672

Product Details anti-CD51/CD61 Antibody

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Reactivity: Human, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51/CD61 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes 110/90 kDa antigen known as the alpha chain of the vitronectin receptor (VNR). Clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines, some epithelia cells. It reacts weakly with platelets. Clone 23C6 recognises the intact complex formed between the CD51 and CD61 molecules (av and b3 integrins). This complex binds vitronectin at the RGD sequence and can also bind von Willebrand factor, fibrinogen, thrombospondin, fibronectin, osteopontin and collagen. Species Reactivity: Human and chicken. Does not cross react with porcine, lapine or bovine tissues.

Immunogen: Human osteoclastoma tissue

Clone: 23C6

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Working dilution of 1:40 -1:80. Recommended second antibodies: FITC-conjugated F(ab')2 rabbit anti-mouse IgG Immunohistochemistry -Frozen sections: Working dilution of 1:50-1:100. Clone 23C6 shows no reactivity on fixed tissues. Immunoprecipitation The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Product: 200 µg purified IgG at approximately 1 mg/mL in PBS at pH 7.4 with 0.09% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD51/CD61

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Alternative Name: CD51/CD61 Complex (ITGAV/ITGB3 Products)