CD56 antibody (FITC)

Catalog No. ABIN187646

Product Details anti-CD56 Antibody (FITC)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: Reacts with human CD56. Species Reactivity: Human, others not tested.

Clone: C5-9

Isotype: IgG2b

Application Details

Application Notes: Immunofluorescence Flow cytometry USE: PBMC: Add 10 µL of mAb/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 4 °C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of mAb/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 ° ° ° °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for lysed whole blood and immunofluorescence analysis with a flow cytometer or microscope. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: FITC labeled monoclonal antibody supplied in 1 mL PBS, 0.08% sodium azide, and 0.2% protein carrier.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56 (NCAM1 Products)

Background: CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells.