Caspase 8 antibody (N-Term)

Catalog No. ABIN187519

Product Details anti-Caspase 8 Antibody

Target: Caspase 8 (CASP8)

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Sterility: 0.2 μm filtered

Immunogen: Recombinant human caspase-8.

Clone: FLICE-1

Isotype: IgG1

Application Details

Application Notes: Western Blot The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mouse monoclonal antibody against human Caspase-8 (cysteine-requiring aspartate protease-8). Available in 100 µl vials at a concentration of 1 mg/ml (100 µg) in PBS with 0.08% sodium azide. Antibody was purified by 0.2 µm sterile filtration.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8 (CASP8 Products)

Background: Caspases are key effectors of programmed cell death. They are synthesized as inactive pro-enzymes, which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker, which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains, which are removed to activate the enzyme along with cleavage in the two subunits. These subunits then form the active protease, which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades