FAS antibody

Catalog No. ABIN187302

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody is specific for human Fas. Species Reactivity: Human. Other species not tested.

Immunogen: Mouse L cells stably over-expressing human Fas.

Clone: 2R2

Isotype: IgG3

Application Details

Application Notes: Functional activity: Induces apoptosis, no cross-linking required. Can be used in DISC (death-inducing signaling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation. Flow cytometry The optimal dilution for a specific application should be determined by the researcher. Figure 1. Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells. Method: (A) Dose response: Jurkat cells (1x105) were treated with agonistic anti-Fas 2R2 antibody at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry. (B) Time course of apoptosis: Jurkat cells (1x105) were treated with 1 µg/mL anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA. Figure 2. Detection of Fas surface expression by FACS analysis. Method: Normal L cells (open histogram) and cells stably over-expressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunostaining was obtained with an isotype matched IgG control.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µg monoclonal antibody at 1 mg/mL purified from concentrated hybridoma tissue culture supernatant. Prepared in PBS containing protein stabilizer. Protein A-affinity purified. Purity 95% by SDS-PAGE.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity