FAS antibody

Catalog No. ABIN187285

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: Reacts specifically with human Fas (APO-1), although the epitope has not been mapped. Species Reactivity: Human. Others not tested.

Immunogen: Human B lymphoblast cell line SKW6.4 cell surface molecules.

Clone: APO-1-1

Isotype: IgG1

Application Details

Application Notes: Functional studies: APO-1-1 will efficiently induce apoptosis if it is used with a cross-linker such as Protein A. The amount of antibody and Protein A needed for any application should be determined by the researcher. Immunoprecipitation: recommend a starting concentration of approximately 1 µg/mL. Western blot Flow cytometry: Use clone APO-1-1 at a 1-2 µg/mL dilution. Immunohistochemistry: APO-1-1 is useful for staining cryostat sections but not for staining on paraffin sections. Immunocytochemistry: Suitable for staining on cytospin preparations. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL of 100 µg/mL monoclonal antibody in PBS. Purity is >95% IgG1 as determined by SDS-gel electrophoresis.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity