TSPO antibody (C-Term)

Catalog No. ABIN185697

Product Details anti-TSPO Antibody

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TSPO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: TSPO / PBR (isoform)

Sequence: RDNHGWRGGR RLPE

Specificity: This antibody is expected to recognise one isoform (NP_000705.2) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RDNHGWRGGRRLPE, from the C Terminus of the protein sequence according to NP_000705.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Colon shows strong cytoplasm staining of absorptive cells. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 17 kDa band observed in Human Breast cancer lysates and approx 16 kDa in Human Ovary and Skin lysates, and in lysates of cell line A431 (calculated MW of 18.8 kDa according to NP_000705.2).These molecular weights are routinely observed

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Alternative Name: TSPO (TSPO Products)

Background: TSPO, PBR, translocator protein (18 kDa) , BZRP, DBI, IBP, MBR, PKBS, PTBR, mDRC, pk18 , OTTHUMP00000028891, benzodiazapine receptor (peripheral), benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor, peripheral benzodiazepine rece

Gene ID: 706

NCBI Accession: NP_000705

Pathways: Cellular Response to Molecule of Bacterial Origin