Ezrin antibody (Internal Region)
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- Target See all Ezrin (EZR) Antibodies
- Ezrin (EZR)
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Ezrin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- Ezrin / villin 2
- Sequence
- QDYEEKTKKA ER
- Cross-Reactivity
- Dog, Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.
- Isotype
- IgG
- Top Product
- Discover our top product EZR Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Colon and Kidney. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 80 kDa band observed in Human Placenta lysates (calculated MW of 69.4 kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Ph
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Ezrin (EZR)
- Alternative Name
- EZR (EZR Products)
- Background
- Villin 2 (ezrin) , ezrin, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584 , cytovillin, villin 2
- Gene ID
- 7430, 22350, 54319
- NCBI Accession
- NP_003370
- Pathways
- Maintenance of Protein Location
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