NET1 antibody (Internal Region)

Catalog No. ABIN185644

Product Details anti-NET1 Antibody

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NET1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: NET1 / ARHGEF8 (Internal)

Sequence: DKLEYLDEKQ RDPR

Specificity: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2, NP_001040625.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80-90 kDa double band in Human Placenta lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Alternative Name: NET1 (NET1 Products)

Background: NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding prot

Gene ID: 10276, 56349, 307098

NCBI Accession: NP_005854, NP_001040625

Pathways: Neurotrophin Signaling Pathway