TRAF1 antibody (C-Term)

Catalog No. ABIN185596

Product Details anti-TRAF1 Antibody

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: TRAF1

Sequence: KLQSPKHAYV KDD

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KLQSPKHAYVKDD, from the C Terminus of the protein sequence according to NP_005649.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus and Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 50-55 kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2 kDa according to NP_005649.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhu

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRAF1

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Alternative Name: TRAF1 (TRAF1 Products)

Background: TRAF1, TNF receptor-associated factor 1 , EBI6, MGC:10353 , Epstein-Bar virus-induced protein 6

Gene ID: 7185, 22029

NCBI Accession: NP_005649

Pathways: NF-kappaB Signaling, Apoptosis, Cell-Cell Junction Organization, Asymmetric Protein Localization